The expressions of EphrinB2 and VEGF in nasopharyngeal carcinoma and their clinical significance / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the expressions of EphrinB2 and VEGF in nasopharyngeal carcinoma and their relationships with clinic pathological factors.@*METHOD@#The expressions of EphrinB2 and VEGF in 136 cases of nasopharyngeal carcinomas, and 20 cases of nasopharyngitis tissues were detected by SP method immunohistochemistry.@*RESULT@#1) The positive rates of EphrinB2 and VEGF were 63.2% and 67.6% respectively in 136 cases of nasopharyngeal carcinomas. Their positive rate in nasopharyneal carcinoma tissues was significantly higher than those in nasopharyngitis tissues (P<0.01). 2) Intensity of the expression of EphrinB2 and VEGF protein was related to lymphnodes metabasis, cranial nerve palsy, basalis encroachment, clinical stage respectively (P<0.01). 3) The expression of EphrinB2 in nasopharyngeal carcinoma tissues and was positively correlated with VEGF (P< 0.01).@*CONCLUSION@#Ephrinbeta2 and VEGF may play important roles in invasiveness, metastasis and angiogenesis of nasopharyngeal carcinoma. There may be certain inter regulation mechanism between them and they are hoped to become new biologic parameters to judge the pathogenesis, development and prognosis of nasopharyngeal carcinoma and to guide the treatment.

Review of research approach on allergens of Shuanghuanglian injection and its important research strategy of susceptibility gene / 中国中药杂志

Reviewing the progress on study about the major allergen of Shuanghuanglian injection in recent years, resulted in that individual differences of anaphylactic shock are closely related with HLA gene polymorphism. Basing on this, we put forward the research strategy on susceptibility gene of important allergen of Shuanghuanglian injection based on the theory of genetic fingerprints, in order to make sure about the relationship the major allergen of Shuanghuanglian injection and HLA-DRB gene polymorphism and specificity IgE antibody, and to clarify the allergic reaction loci reduced allergic reactions, which can provide the reference data for the study on mechanisms for anaphylactic reaction of Shuanghuanglian injection, and research ideas for the sensitization mechanism of traditional Chinese medicine injection study.

Clinical study on changes of serum levels of immunoglobulin and cytokines in anaphylaxis patients induced by Qingkailing injection / 中国中药杂志

<p><b>OBJECTIVE</b>To determine the relation between the change of immunology indexes of serum and the occurrence of anaphylaxis in patients induced by Qingkailing injection, and to establish the method of detection of the type of anaphylaxis in patients induced by traditional Chinese medicine (TCM) injection.</p><p><b>METHOD</b>In a prospective nested case-control study, we compared the concentration of IgE, IgG, IL-2, IL-4 of 20 cases in anaphylaxis induced by Qingkailing injection with those from 60 matched controls who remained normal after the using Qingkailing injection. The correlation between the change of IgE, IgG, IL-2, IL-4 of serum and the occurrence of anaphylaxis in patients induced by Qingkailing injection was determined, and we also evaluate the clinical values of IgE, IgG, IL-2, IL-4 of serum in diagnosis of anaphylaxis in patients induced by Qingkailing injection by Binary-Logistic regression analysis.</p><p><b>RESULT</b>The concentrations of IgE, IgG, IL-2, IL-4 of serum of 20 cases were significantly higher than those in controls (P < or = 0. 05). The concentrations of IgE, IgG, IL-2, IL4 of serum of 20 cases after the treatment with Qingkailing injection were significantly higher than those before the treatment with Qingkailing injection (P < or = 0.05). The Exp(B) of IgE, IgG, IL-2, IL-4 were 22. 018 (95.0% CI for EXP was 3.268-310.831), 11.271 (95.0% CI for EXP was 1.91-3.024), 23.612 (95.0% CI for EXP was 3.012-421.187), 16.486 (95.0% CI for EXP was 1.233-205.413), respectively.</p><p><b>CONCLUSION</b>The allergic reaction caused by Qingkailing injection may be due to type I allergic reactions; The presence of IgE, IgG, IL-2, IL-4 can serve as clinical evaluation indicators in evaluation the damage degree of immune system caused by anaphylaxis in patients induced by Qingkailing injection. Cytokines are more likely to become the effective evaluation index in diagnosis of anaphylaxis in patients induced by TCM injection.</p>

Correlation between change of immunotoxicology indexes of cerum and the occurrence of anaphylaxis in patients induced by qingkailing injection: a clinical study / 中国中药杂志

<p><b>OBJECTIVE</b>To determine the relation between the change of immunotoxicology indexes of serum and the occurrence of anaphylaxis in patients induced by Qingkailing injection, which is composed by cholic acid, mother-of-pearl, gardenia, cornu bubali, isatis root, baicalin, honeysuckle, and to establish the method of detection of the type of anaphylaxis in patients induced by traditional Chinese medicine (TCM) injection.</p><p><b>METHOD</b>In a prospective nested case-control study, we compared the concentration of IgE, IgG, IL-4, histamine of 18 cases in anaphylaxis induced by Qingkailing injection with those from 72 matched controls who remained normal after the using Qingkailing injection. The correlation between the change of IgE, IgG, IL-4, histamine of serum and the occurrence of anaphylaxis in patients induced by Qingkailing injection was determined, and we also evaluate the clinical values of IgE, IgG, IL-4, histamine of serum in diagnosis of anaphylaxis in patients induced by Qingkailing injection by receiver operating characteristic (ROC) curves.</p><p><b>RESULT</b>The concentrations of IgE, IgG, IL-4, histamine of serum of 18 cases were significantly higher than those in controls (P<0.05), the area under the curve (AUC) of IL-4, IgG, IgE, histamine were 0.362 (95.0% CI. for EXP was 0.978-2.405), 0.349 (95.0% CI. for EXP was 1.448-316.232), 0.350 (95.0% CI. for EXP was 1.262-601.306), 0.374 (95.0% CI. for EXP was 1.003-45.413), respectively.</p><p><b>CONCLUSION</b>The allergic reaction caused by Qingkailing injection may be due to type I allergic reactions; The presence of IgE, IgG, IL-4, Histamine can serve as clinical evaluation indicators in evaluation the damage degree of immune system caused by anaphylaxis in patients induced by Qingkailing injection. Cytokines are more likely to become the effective evaluation index in diagnosis of anaphylaxis in patients induced by TCM injection.</p>

Application of nested case-control study on safe evaluation of post-marketing traditional Chinese medicine injection / 中国中药杂志

The nested case-control study design (or the case-control in a cohort study) is described here as a new study design used in safe evaluation of post-marketing traditional Chinese medicine injection. In the nested case-control study, cases of a disease that occur in a defined cohort are identified and, for each, a specified number of matched controls is selected from among those in the cohort who have not developed the disease by the time of disease occurrence in the case. For many research questions, the nested case-control design potentially offers impressive reductions in costs and efforts of data collection and analysis compared with the full cohort approach, with relatively minor loss in statistical efficiency. The nested case-control design is particularly advantageous for studies in safe evaluation of post-marketing traditional Chinese medicine injection. Some examples of the application of nested case-control study were given.

Strategy of individualized screening of allergens in serum of patients allergic to Shuanghuanglian injection based on RBL-2H3 mast cell model / 中国中药杂志

Screening of allergens in Shuanghuanglian injection is now one of the key and urgently solved problems in assessment on the safety of traditional Chinese medicine injections. In this paper, the author reviewed the improtant progresses in study on Screening of allergens in shuanghuanglian injection. The principle, concrete methods and specific procedure were discussed on the strategy of individualized screening of allergens in serum of patients allergic to Shuanghuanglian injection based on RBL-2H3 mast cell model.

Clinical re-evaluation of effects of different treatments to prevent from phlebitis induced by Chansu injection / 中国中药杂志

<p><b>OBJECTIVE</b>To re-evaluate the effects of different treatments to prevent from phlebitis induced by Chansu injection.</p><p><b>METHOD</b>Patients treated with Chansu injection were divided randomly into 4 groups with 50 per group, control group, the magnesium sulfate group, phentolaminum group, and anisodamine group. Patients in the control group only received the routine nursing treatment, and patients in the various experiment group received different interventions. The comparison was made in the morbidity and the starting time of occurrence of phlebitis, the severity of pain, duration of pain.</p><p><b>RESULT</b>The morbidity of phlebitis was 8%, 8%, 6% respectively. The starting time of phlebitis occurrence was (21 +/- 9.31) , (22.34 +/- 10.15), (20.19 +/- 11.23) h, respectively. The NRS of pain was (4. 15 +/- 1.03), (3.26 +/- 1.17), (4.32 +/- 1.36), respectively. The duration time of pain was (4.05 +/- 1.21), (3.37 +/- 1.17), (3.19 +/- 1.67) d, respectively. In control group, the morbidity of phlebitis, the starting time of occurrence of phlebitis, the severity of pain, duration of pain was 24%, (17 +/- 6.32) h, (6.58 +/- 1.29), (5.32 +/- 1.12) d, respectively. As compared with the control group, a significance difference was found between every group in three test groups and control group respectively (P<0.05).</p><p><b>CONCLUSION</b>The morbidity and the starting time of occurrence of phlebitis, the severity of pain, duration of pain was significantly reduced respectively by external appication of magnesium sulfate, anisodamine, and intravenous drip infusion of phentolaminum.</p>

Expressions of EphA2 and EphrinA1 in bladder carcinomas and their clinical significance / 中国医师进修杂志

Objective To investigate the expressions of EphA2 and EphrinA1 in bladder carcinomas and their clinical significance. Method The expressions of EphA2 and EphrinA1 were detected on bladder carcinomas (75 cases ) by immunohistochemistry S-P method. Results ( 1 ) The positive rates of expression of EphA2 and EphrinA1 were 61.3%(46/75) and 46.7%(35/75) respectively. (2) The expression of EphA2 was related to tumor size, lymphatic metastasis and TNM staging(P＜ 0.05),but not to gender,age and pathology type (P ＞ 0.05 ). ( 3 )The expression of EphrinA1 was related to tumor size and lymphatic metastasis (P ＜ 0.05 ), but not to gender, age, pathology type and TNM staging (P ＞ 0.05 ). (4)There was significantly positive correlation between the expression of EphA2 and EphrinA1 (P ＜ 0.05).Conclusions EphA2 and EphrinA1 are related to the occurrence and development of bladder carcinoma.The combined detection of EphA2 and EphrinA1 can help to predict the clinical and pathologic characteristics of bladder carcinoma. It is an important index to assist treatment, they are predictive factors for bladder carcinoma.

One-stage reconstruction of anterior/posterior cruciate ligaments using the allogenic achilles tendon in 3 cases / 中国组织工程研究

To explore the effect of allogenic achilles tendon on one-stage reconstruction of antedor/postedor cruciate ligaments,3 cases with ruptured anterior/posterior cruciate ligaments and received allogenic achilles tendon reconstruction,at Department of Orthopaedics,General Hospital of Chinese People's Armed Police Forces,from June 207 to April 2008,were collected.A more than 6-month follow-up showed that all cases could walk at 12 weeks postoperatively.Anterior andposterior drawing tests,as well as inversion and extroversion stress test were negative,with 100° movement ranges.In addition,no serious complication occurred.The Lysholm knee fuction scores were 87,83 and 85,respectively.The results suggest that the allogenic achilles tendon seems to be a satisfactory treatment option for one-stage anterior/posterior cruciate ligaments reconstruction,which has merits of easy operation,small trauma and good short-term results.However,further research is needed to determine the role of allogenic achilles tendon in knees with multi-ligament injury.

Tracing eyeball movements in Parkinson' s disease / 中华神经科杂志

Objective To evaluate the eyeball movement examination on Parkinson' s disease (PD) and investigate their vestibular function.Methods Thirty healthy volunteers and 55 PD patients served as subjects.Thirty-one patients had bradykinesia, 23 patients had resting tremor at first visit and 1 had both.Electronystagmogram examinations were performed in all the subjects.The eyeball movment gain (G) of PD group was compared with controls and the difference between the bradykinesia group and resting tremor group was analyzed.Dissymmetry of the bilateral pursuit movement was investigated.Results The eyeball movement gains decreased in PD patients (Left G 0.79±0.10, Right G 0.78±0.11) significantly (U = 394.500, t = 3.547, P < 0.01) compared with controls (Left G 0.86±0.05, Right G 0.85±0.06).There was no difference (t = -0.100,2.005, P0.05) between the bradykinesia group (Left G 0.79± 0.14,Right G 0.74±0.14) and the resting tremor group ( Left G 0.79±0.06,Right G 0.81±0.07).The dissymmetry of the bilateral movement increased significantly (U = 755.500, P < 0.05) in PD group (1.03±0.97) than in controls (0.87±0.86).Conclusion The velocity of the eyeball movement has decreased in patients with PD.There is no difference of the pursuit gain between bradykinesia group and the resting tremor group.The bilateral vestibular function is involved dissymmetrically.

Alternation of Saccadic System in Patients with Parkinson's Disease(review) / 中国康复理论与实践

@#The saccadic movement of patients with Parkinson's disease was abnormal and might be related to the severity of the disease.The abnormal saccadic movement was not related to the symptoms of Parkinson's disease directly,the accesses of controlling limbs' and eyes' movement are comparatively independent.

Study of the eye movements system of the patients with vertebral-basilar insufficiency / 中国康复理论与实践

@# ObjectiveTo investigate the characteristic and the function of the eye movements system of the patients with vertebral-basilar insufficiency (VBI).Methods83 patients with VBI and 57 healthy controls were tested with electronystagmogram instrument,include saccade test, Eye Tracking Test (ETT) and optokinetic test(OKN).Results and ConclusionDelay lower accuracy of saccade test, decreased gain of OKN, asymmetry of horizontal ETT were showed in the patients with VBI.